Journal: Journal of fungi (Basel, Switzerland)

Article Title: TtCel7A: A Native Thermophilic Bifunctional Cellulose/Xylanase Exogluclanase from the Thermophilic Biomass-Degrading Fungus Thielavia terrestris Co3Bag1, and Its Application in Enzymatic Hydrolysis of Agroindustrial Derivatives.

doi: 10.3390/jof9020152

Figure Lengend Snippet: Figure 2. Purification and zymogram analysis of TtCel7A on 10% SDS-PAGE. (a) A 10% SDS-PAGE analysis of purified TtCel7A. Lanes M: molecular weight standard; 1: crude extract; 2: anion exchange chromatography fractions; 3: purified TtCel7A. (b) Zymogram analysis of TtCel7A using 1% CMC as substrate. (c) Zymogram analysis of TtCel7A, using 1% RBB-X as substrate.

Article Snippet: The dialyzed protein was loaded onto an anion exchange chromatography column (UNOsphere Q, Bio-Rad, USA).

Techniques: SDS Page, Molecular Weight, Chromatography

Journal: NPJ Biofilms and Microbiomes

Article Title: Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearance

doi: 10.1038/s41522-023-00433-0

Figure Lengend Snippet: The levels of cAMP a and cGMP b in the colon, and the activity of adenylyl cyclase c and guanylyl cyclase d ; data were analyzed with two-tailed Student’s t-test and presented as mean ± SEM. n = 6. * P < 0.05. e Western blots showing the protein expression of SLC9A3, SLC5A1, SLC26A3, and GAPDH in the colon; cAMP content f , adenylyl cyclase activity g , cGMP content h , and guanylyl cyclase activity i in the primary colonocytes; j Western blots showing the protein expression of SLC9A3, SLC5A1, SLC26A3 and GAPDH in the primary colonocytes; Relative level of Na + k and Cl - l in the culture medium. Data were analyzed using one-way analysis of variance (ANOVA) and presented as the mean ± SEM. n = 4. * P < 0.05, ** P < 0.01.

Article Snippet: The membranes were blocked with 5% skim milk and then incubated with antibodies against SLC9A3 (Bioss Cat#bs-8601R; RRID: AB_2928052; Dilution, 1:500), SLC5A1 (Bioss, Cat#bs-1128R; RRID: AB_10856441; Dilution, 1:500), SLC26A3 (Boster, Cat#A03335; RRID: not found; Dilution, 1:1000), and GAPDH (Abcam Cat# ab8245; RRID: AB_2107448; Dilution, 1:2000) overnight and then with secondary antibodies for 2 h at 22 ± 2 °C.

Techniques: Activity Assay, Two Tailed Test, Western Blot, Expressing